Midv-679

Steps:

| Test | Principle | Turn‑around time | Sensitivity / Specificity | |------|-----------|------------------|---------------------------| | | Targeted amplification of the G‑gene unique to MIDV‑679 | 4‑6 h (lab‑based) | 92 % / 98 % (validated on 483 specimens) | | Serology (IgM/IgG ELISA) | Recombinant G‑protein antigen | 1‑2 days (batch) | IgM 88 % / IgG 95 % after day 7 of symptoms | | Virus isolation (Vero cells) | Cytopathic effect; confirm by immunofluorescence | 5‑7 days | Gold standard but low throughput | | Metagenomic NGS | Unbiased sequencing of clinical specimens | 24‑48 h (cloud‑based pipeline) | Detects co‑infections; useful for atypical cases | MIDV-679