To ensure your ISO is a "perfect" rip without corruption, you should verify its MD5 or SHA-1 hash against the database.
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“Invalid ISO” or Dolphin won’t boot. Solution: Your ISO is corrupted or compressed incorrectly. GameCube ISOs must not be zipped. If it has a .nkit extension, you need to convert it back to .iso using the NKit tool. Dolphin prefers full, raw ISOs. To ensure your ISO is a "perfect" rip
This guide is for only.